One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.
نویسندگان
چکیده
We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 78 15 شماره
صفحات -
تاریخ انتشار 2012